4LGI
N-terminal truncated NleC structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.512, 88.664, 110.653 |
| Unit cell angles | 90.00, 92.89, 90.00 |
Refinement procedure
| Resolution | 37.607 - 2.301 |
| R-factor | 0.2541 |
| Rwork | 0.253 |
| R-free | 0.28020 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.226 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXS |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 98250 | |
| Completeness [%] | 98.1 | 98.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 6 | 291 | 0.2M (NH4)2SO4, 100mM MES pH 6.0, 20%(w/v) polyethylene glycol 3000, EVAPORATION, temperature 291K |
