4LG6
Crystal structure of ANKRA2-CCDC8 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2011-11-09 |
Detector | RIGAKU RAXIS |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 38.726, 49.791, 41.243 |
Unit cell angles | 90.00, 98.23, 90.00 |
Refinement procedure
Resolution | 26.149 - 1.800 |
R-factor | 0.197 |
Rwork | 0.196 |
R-free | 0.20850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3so8 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.352 |
Data reduction software | XDS |
Data scaling software | SCALA (3.3.20) |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.818 | 26.135 | 1.900 |
High resolution limit [Å] | 1.800 | 5.690 | 1.800 |
Rmerge | 0.020 | 0.650 | |
Total number of observations | 1688 | 7328 | |
Number of reflections | 14082 | ||
<I/σ(I)> | 16.2 | 24.8 | 1.2 |
Completeness [%] | 97.0 | 98.5 | 94.8 |
Redundancy | 3.7 | 3.5 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 291 | 25% PEG3350, 0.2 M sodium chloride, 0.1 M HEPES, pH 7.5, vapor diffusion, temperature 291K |