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4LFA

Crystal Structure of 30S ribosomal subunit from Thermus thermophilus

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 24-ID-C
Synchrotron siteAPS
Beamline24-ID-C
Temperature [K]100
Detector technologyCCD
Collection date2010-11-07
DetectorADSC QUANTUM 315
Wavelength(s)0.98
Spacegroup nameP 41 21 2
Unit cell lengths402.314, 402.314, 175.651
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution34.853 - 3.646
R-factor0.1561
Rwork0.153
R-free0.20430
Structure solution methodFOURIER SYNTHESIS
RMSD bond length0.010
RMSD bond angle1.538
Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwarePHENIX
Refinement softwarePHENIX (dev_1119)
Data quality characteristics
 OverallInner shellOuter shell
Low resolution limit [Å]35.00035.0003.780
High resolution limit [Å]3.6467.8403.646
Rmerge0.0630.0280.708
Number of reflections154622
<I/σ(I)>13.5
Completeness [%]97.690.399.5
Redundancy3.13.13.1
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1hanging drop6.5277MPD, pH 6.5, hanging drop, temperature 277K

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