4LF9
Crystal Structure of 30S ribosomal subunit from Thermus thermophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 402.042, 402.042, 176.388 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.475 - 3.281 |
| R-factor | 0.1704 |
| Rwork | 0.169 |
| R-free | 0.20120 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.771 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX (dev_1119) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 35.000 | 35.000 | 3.400 |
| High resolution limit [Å] | 3.280 | 7.050 | 3.280 |
| Rmerge | 0.093 | 0.056 | 0.911 |
| Number of reflections | 216436 | ||
| <I/σ(I)> | 14.7 | ||
| Completeness [%] | 99.1 | 95.8 | 99.3 |
| Redundancy | 3.9 | 3.7 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 6.5 | 277 | MPD, pH 6.5, hanging drop, temperature 277K |
