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4LF8

Crystal Structure of 30S ribosomal subunit from Thermus thermophilus

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 24-ID-C
Synchrotron siteAPS
Beamline24-ID-C
Temperature [K]100
Detector technologyCCD
Collection date2010-11-07
DetectorADSC QUANTUM 315
Wavelength(s)0.98
Spacegroup nameP 41 21 2
Unit cell lengths402.445, 402.445, 176.924
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution34.615 - 3.148
R-factor0.1701
Rwork0.168
R-free0.20540
Structure solution methodFOURIER SYNTHESIS
RMSD bond length0.004
RMSD bond angle0.816
Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwarePHENIX
Refinement softwarePHENIX (dev_1119)
Data quality characteristics
 OverallInner shellOuter shell
Low resolution limit [Å]35.00035.0003.260
High resolution limit [Å]3.1486.7703.148
Rmerge0.0780.0280.677
Number of reflections243896
<I/σ(I)>13.3
Completeness [%]98.392.899.1
Redundancy3.33.23.3
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1hanging drop6.5277MPD, pH 6.5, hanging drop, temperature 277K

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