4LF6

Crystal Structure of 30S ribosomal subunit from Thermus thermophilus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2010-11-07
Detector	ADSC QUANTUM 315
Wavelength(s)	0.98
Spacegroup name	P 41 21 2
Unit cell lengths	403.178, 403.178, 176.492
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.895 - 3.305
R-factor	0.1772
Rwork	0.175
R-free	0.21040
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.004
RMSD bond angle	0.804
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (dev_1119)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.000	35.000	3.420
High resolution limit [Å]	3.300	7.090	3.300
Rmerge	0.104	0.033	0.775
Number of reflections	212335
<I/σ(I)>	8.6
Completeness [%]	98.8	95.4	99
Redundancy	4	3.9	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	hanging drop	6.5	277	MPD, pH 6.5, hanging drop, temperature 277K