4LF2
Hexameric Form II RuBisCO from Rhodopseudomonas palustris, activated and complexed with sulfate and magnesium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-01-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 74.221, 99.777, 100.000 |
| Unit cell angles | 67.30, 71.28, 86.63 |
Refinement procedure
| Resolution | 87.430 - 2.380 |
| R-factor | 0.2053 |
| Rwork | 0.203 |
| R-free | 0.25760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.462 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (1.3.1) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 90.000 | 90.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 3.990 | 1.850 |
| Rmerge | 0.139 | 0.110 | 0.754 |
| Number of reflections | 184523 | ||
| <I/σ(I)> | 10.3 | ||
| Completeness [%] | 85.4 | 97.5 | 39.1 |
| Redundancy | 3.5 | 3.7 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 170 mM lithium sulfate, 85 mM Tris-HCl, 24 % PEG 4000, 14 % glycerol, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
