4LF1
Hexameric Form II RuBisCO from Rhodopseudomonas palustris, activated and complexed with 2-CABP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 75.033, 100.720, 100.690 |
| Unit cell angles | 66.51, 108.32, 95.41 |
Refinement procedure
| Resolution | 71.290 - 2.380 |
| R-factor | 0.2658 |
| Rwork | 0.263 |
| R-free | 0.31530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.577 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR (2.5) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 80.000 | 80.000 | 2.470 |
| High resolution limit [Å] | 2.380 | 5.130 | 2.380 |
| Rmerge | 0.179 | 0.102 | 0.563 |
| Number of reflections | 101179 | ||
| <I/σ(I)> | 6.4 | ||
| Completeness [%] | 98.1 | 99.4 | 97 |
| Redundancy | 3.3 | 3.3 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 60 mM Tris-HCl, 20 % (w/w) PEG 4000, and 10 % (v/v) glycerol , pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
