4LEW
Structure of human cGAS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 153 |
Detector technology | CCD |
Collection date | 2013-02-23 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.815, 111.480, 76.414 |
Unit cell angles | 90.00, 92.73, 90.00 |
Refinement procedure
Resolution | 40.747 - 2.040 |
R-factor | 0.1805 |
Rwork | 0.179 |
R-free | 0.22280 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.122 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.100 | 2.080 |
High resolution limit [Å] | 2.040 | 2.040 |
Rmerge | 0.083 | |
<I/σ(I)> | 31.2 | |
Completeness [%] | 99.7 | 99.5 |
Redundancy | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 15-20% PEG 3350, 0.2 M A.S., pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |