4LEJ
Crystal Structure of the Korean pine (Pinus koraiensis) vicilin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 110 |
Detector technology | CCD |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | I 21 3 |
Unit cell lengths | 148.780, 148.780, 148.780 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.268 - 2.402 |
R-factor | 0.1947 |
Rwork | 0.192 |
R-free | 0.24140 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.802 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.2_1304) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.440 |
High resolution limit [Å] | 2.400 | 6.510 | 2.400 |
Rmerge | 0.106 | 0.108 | 0.752 |
Number of reflections | 21280 | ||
<I/σ(I)> | 20 | ||
Completeness [%] | 98.9 | 81.5 | 100 |
Redundancy | 7.2 | 5.5 | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 2M sodium formate, 0.1 M sodium acetate trihydrate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |