4LED
The Crystal Structure of Pyocin L1 bound to D-rhamnose at 2.37 Angstroms
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9788 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 52.990, 160.650, 150.570 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.930 - 2.370 |
| R-factor | 0.21128 |
| Rwork | 0.209 |
| R-free | 0.25685 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4le7 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.686 |
| Data reduction software | xia2 |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.930 | 2.430 |
| High resolution limit [Å] | 2.370 | 2.370 |
| Rmerge | 0.059 | 0.830 |
| Number of reflections | 26242 | |
| <I/σ(I)> | 19 | 2.1 |
| Completeness [%] | 99.1 | 99.5 |
| Redundancy | 4.4 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 20% PEG 550 MME, 20% PEG 20K, 0.03 M CaCl2, 0.03 M MgCl2 0.1 M MOPS/HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
