4LEC
Human Methyltransferase-Like Protein 21A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-05-31 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 31 |
| Unit cell lengths | 72.166, 72.166, 102.954 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.080 - 2.280 |
| R-factor | 0.22 |
| Rwork | 0.219 |
| R-free | 0.25560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bzb |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.160 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP (11.0) |
| Refinement software | BUSTER-TNT (BUSTER 2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.320 |
| High resolution limit [Å] | 2.280 | 2.280 |
| Rmerge | 0.112 | 0.938 |
| Number of reflections | 27200 | |
| <I/σ(I)> | 19.5 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.6 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 15% PEG 3350, 0.1 M Succinate Acid, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
