4LDX
Crystal structure of the DNA binding domain of arabidopsis thaliana auxin response factor 1 (ARF1) in complex with protomor-like sequence ER7
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-09-08 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9334 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 43.520, 105.190, 127.910 |
Unit cell angles | 90.00, 98.14, 90.00 |
Refinement procedure
Resolution | 33.810 - 2.900 |
R-factor | 0.21626 |
Rwork | 0.214 |
R-free | 0.25932 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ldv |
RMSD bond length | 0.011 |
RMSD bond angle | 1.502 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 3.060 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.160 | 0.634 |
Number of reflections | 25033 | |
<I/σ(I)> | 6.2 | 1.7 |
Completeness [%] | 98.7 | 98.8 |
Redundancy | 2.6 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 100 nL complex (4 mg/mL ARF1) and 100 nL crystallization buffer (10% PEG 20K, Glycine pH 8.5, 7.5% propanediol), VAPOR DIFFUSION, SITTING DROP, temperature 293K |