4LDW
Crystal structure of the DNA Binding Domain of arabidopsis thaliana auxin response factor 1, P21 structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9393 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.260, 83.670, 79.027 |
| Unit cell angles | 90.00, 117.61, 90.00 |
Refinement procedure
| Resolution | 35.940 - 2.670 |
| R-factor | 0.21885 |
| Rwork | 0.216 |
| R-free | 0.27945 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ldv |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.481 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.020 | 2.810 |
| High resolution limit [Å] | 2.670 | 2.670 |
| Rmerge | 0.098 | 0.650 |
| Number of reflections | 23332 | |
| <I/σ(I)> | 6.9 | 2.2 |
| Completeness [%] | 96.6 | 96.2 |
| Redundancy | 3.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 1 4 mg/mL ARF1DBD + 1 l crystallization buffer (100 mM MES 6.5, 20%PEG 5K MME) 7.7.11), VAPOR DIFFUSION, SITTING DROP, temperature 293K |
