4LDU
Crystal structure of the DNA binding domain of Arabidopsis thaliana auxin response factor 5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-05-16 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.93340 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 69.613, 91.502, 77.182 |
Unit cell angles | 90.00, 114.28, 90.00 |
Refinement procedure
Resolution | 38.380 - 2.150 |
R-factor | 0.19771 |
Rwork | 0.195 |
R-free | 0.25206 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 1.626 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.000 | 2.270 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.063 | 0.430 |
Number of reflections | 23983 | |
<I/σ(I)> | 11 | 2.9 |
Completeness [%] | 99.8 | 100 |
Redundancy | 2.8 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 100 nL 13 mg/mL ARF5DBD +100 nl crystallization buffer (20 mM TRIS 7.5, 12.5% PEG MME 2000), VAPOR DIFFUSION, SITTING DROP, temperature 293K |