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4LDJ

Crystal Structure of a GDP-bound G12C Oncogenic Mutant of Human GTPase KRas

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 19-ID
Synchrotron siteAPS
Beamline19-ID
Temperature [K]100
Detector technologyCCD
Collection date2013-01-01
DetectorADSC QUANTUM 315
Wavelength(s)0.97918
Spacegroup nameP 21 21 21
Unit cell lengths39.071, 40.724, 91.431
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution20.696 - 1.150
R-factor0.1329
Rwork0.132
R-free0.16220
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)4epv
RMSD bond length0.015
RMSD bond angle1.674
Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwarePHASER (2.5.3)
Refinement softwarePHENIX (1.8.2_1309)
Data quality characteristics
 OverallInner shellOuter shell
Low resolution limit [Å]50.00050.0001.170
High resolution limit [Å]1.1503.1201.150
Rmerge0.0750.0630.317
Number of reflections52661
<I/σ(I)>9.630.072.48
Completeness [%]99.699.294.8
Redundancy77.33
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP8.52930.2M Sodium Acetate pH4.5, 0.1M Tris pH 8.5, 28% PEG 3350, Vapor Diffusion, Hanging Drop, temperature 293K, VAPOR DIFFUSION, HANGING DROP

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