4LDD

Crystal Structure of Ebola virus VP40 Hexamer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	85
Detector technology	CCD
Collection date	2012-08-19
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97945
Spacegroup name	P 4 21 2
Unit cell lengths	134.479, 134.479, 136.087
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.885 - 3.498
R-factor	0.3071
Rwork	0.306
R-free	0.32250
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.719
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: dev_1150))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	136.087
High resolution limit [Å]	3.497	3.497
Number of reflections	16180
Completeness [%]	98.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	0.1 M bicine, 15% PEG20000, 3% dextran sulfate sodium salt (Mr = 5 kDa), pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K