4LD1
Structural analysis of the microcephaly protein CPAP G-box domain suggests a role in centriole elongation.
Experimental procedure
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-03-16 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 41.632, 50.254, 60.345 |
Unit cell angles | 90.00, 108.56, 90.00 |
Refinement procedure
Resolution | 39.467 - 1.440 |
R-factor | 0.1549 |
Rwork | 0.154 |
R-free | 0.17970 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.204 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX (AUTOSOL) |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 39.470 |
High resolution limit [Å] | 1.440 |
Number of reflections | 42577 |
Completeness [%] | 99.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 0.1 M MES/Imidazole, 0.03 M sodium nitrate, 0.03 M disodium hydrogen phosphate, 0.03 M ammonium sulfate, 12.5% w/v PEG 1000, 12.5% w/v PEG 3350, 12.5% v/v MPD , VAPOR DIFFUSION, SITTING DROP, temperature 277K, pH 6.5 |