4LCY
Crystal structure of HLA-b46 at 1.6 angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2012-07-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.950, 89.730, 94.620 |
| Unit cell angles | 90.00, 98.45, 90.00 |
Refinement procedure
| Resolution | 25.200 - 1.600 |
| R-factor | 0.17383 |
| Rwork | 0.172 |
| R-free | 0.20111 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3c9n |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.535 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.200 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.049 | 0.166 |
| Number of reflections | 107515 | |
| <I/σ(I)> | 12.8 | 5.7 |
| Completeness [%] | 99.0 | 99 |
| Redundancy | 3.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 0.1M BIS-TRIS pH 5.5, 25% POLYETHYLENE GLYCOL 3350 , VAPOR DIFFUSION, HANGING DROP, temperature 298K |
