4LCX
The structure of hemagglutinin from avian-origin H7N9 influenza virus (A/Shanghai/1/2013)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-05-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.880, 225.982, 75.336 |
| Unit cell angles | 90.00, 117.92, 90.00 |
Refinement procedure
| Resolution | 49.882 - 3.094 |
| R-factor | 0.2283 |
| Rwork | 0.226 |
| R-free | 0.26590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.343 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.210 |
| High resolution limit [Å] | 3.094 | 3.100 |
| Number of reflections | 39468 | |
| Completeness [%] | 99.6 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 18% PEG 8000, 18% Ethylene glycol, 0.12 M Di-Ethyleneglycol, 0.12 M Tri-Ethyleneglycol, 0.12 M TetraEthyleneglycol, 0.12 M Penta-Ethyleneglycol, 0.1 M Tris, 0.1 M Bicine, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
