4LCT
Crystal Structure and Versatile Functional Roles of the COP9 Signalosome Subunit 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 202.210, 87.090, 133.600 |
| Unit cell angles | 90.00, 105.47, 90.00 |
Refinement procedure
| Resolution | 48.700 - 2.700 |
| R-factor | 0.2259 |
| Rwork | 0.224 |
| R-free | 0.27180 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.317 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.700 | |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 61556 | |
| Completeness [%] | 26.7 | 99.73 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 293.15 | 0.1M BTP pH 7.0, 0.2M ammonium sulfate, 10% (w/v) polyethylene glycol 8000, 5mM dithiothreitol, VAPOR DIFFUSION, temperature 293.15K |
