4LCI
Anti canine CD28 antibody, 1C6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-03 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.599, 71.621, 73.234 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.600 - 1.900 |
| R-factor | 0.18157 |
| Rwork | 0.179 |
| R-free | 0.23038 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1h8n |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.584 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.600 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.255 | |
| Number of reflections | 18024 | |
| <I/σ(I)> | 4.1 | |
| Completeness [%] | 91.1 | 94.99 |
| Redundancy | 1.38 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 3.5M Sodium formate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
