4LCB
Structure of Vps4 homolog from Acidianus hospitalis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-01-05 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9795 |
Spacegroup name | P 65 |
Unit cell lengths | 95.962, 95.962, 79.499 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 2.080 |
R-factor | 0.2156 |
Rwork | 0.213 |
R-free | 0.25430 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4lgm |
RMSD bond length | 0.017 |
RMSD bond angle | 1.442 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.1.4) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 2.180 |
High resolution limit [Å] | 2.100 | 4.520 | 2.100 |
Rmerge | 0.083 | 0.055 | 0.673 |
Number of reflections | 24654 | ||
<I/σ(I)> | 17.2 | ||
Completeness [%] | 99.1 | 91.2 | 100 |
Redundancy | 9.3 | 8.3 | 9.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.2 M MgCl2, 0.1 M HEPES pH 7.5, 30 % PEG400, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K |