4LBE
Structure of KcsA with R122A mutation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 193 |
Detector technology | CCD |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.1000 |
Spacegroup name | I 4 |
Unit cell lengths | 155.938, 155.938, 75.814 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.871 - 2.751 |
R-factor | 0.169 |
Rwork | 0.167 |
R-free | 0.20180 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.819 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 100.000 | 100.000 | 2.850 |
High resolution limit [Å] | 2.750 | 5.920 | 2.750 |
Rmerge | 0.083 | 0.029 | 0.655 |
Number of reflections | 23784 | ||
<I/σ(I)> | 9.9 | ||
Completeness [%] | 99.9 | 99.5 | 99.2 |
Redundancy | 7 | 7 | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | PEG 400, MgAcetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP |