4L9V
Crystal structure of Se-Met derivative MepR F27L mutant from multidrug resistant S. aureus clinical isolate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-28 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97915 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 120.767, 120.767, 54.476 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.604 - 2.374 |
| R-factor | 0.2337 |
| Rwork | 0.231 |
| R-free | 0.25630 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.637 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX ((Phaser)) |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.374 |
| Number of reflections | 6201 |
| Completeness [%] | 99.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 293 | 0.2 M Na tartrate dibasic, 20% PEG 3350, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
