4L9U

Structure of C-terminal coiled coil of RasGRP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-05-27
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0000
Spacegroup name	P 21 21 2
Unit cell lengths	24.878, 165.047, 28.319
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.911 - 1.601
R-factor	0.2024
Rwork	0.201
R-free	0.23510
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.146
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	27.911	27.910	1.630
High resolution limit [Å]	1.600	8.470	1.600
Number of reflections	16098
Completeness [%]	98.9	89.7	97.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	3.6	296	20 mM sodium acetate, 22% PEG 3350, 100 mM lithium sulfate, 0.4% formamide, pH 3.6, VAPOR DIFFUSION, SITTING DROP, temperature 296K