4L9T
Crystal structure of MepR F27L mutant from multidrug resistant S. aureus clinical isolate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-28 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.441, 113.122, 49.936 |
| Unit cell angles | 90.00, 98.18, 90.00 |
Refinement procedure
| Resolution | 24.720 - 1.794 |
| R-factor | 0.1717 |
| Rwork | 0.170 |
| R-free | 0.20340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.894 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX ((Phaser)) |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 25.000 |
| High resolution limit [Å] | 1.790 |
| Number of reflections | 32048 |
| Completeness [%] | 96.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.2 M (NH4)2HPO4, 20% PEG 3350, pH 8, vapor diffusion, hanging drop, temperature 293K |
