4L9J
Crystal structure of S. aureus MepR in DNA-binding conformation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-06-11 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97236 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 98.806, 98.806, 151.520 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.040 - 3.450 |
R-factor | 0.2288 |
Rwork | 0.225 |
R-free | 0.26430 |
Structure solution method | SAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.649 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX ((Phaser)) |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 3.450 |
Number of reflections | 10448 |
Completeness [%] | 99.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 2 mM CuCl2, 50 mM Tris HCl pH 8.5, 1.8 M Li2SO4, and 0.5 mM spermine, VAPOR DIFFUSION, HANGING DROP, temperature 293K |