4L9H

Crystal structure of the FP domain of human F-box protein Fbxo7(SeMet)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97872
Spacegroup name	P 43 21 2
Unit cell lengths	57.650, 57.650, 89.320
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.765 - 2.000
R-factor	0.2048
Rwork	0.201
R-free	0.23450
RMSD bond length	0.004
RMSD bond angle	0.879
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall
Low resolution limit [Å]	99.991
High resolution limit [Å]	2.000
Number of reflections	10718

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	PEG 3350, MOPS 7.0, KBr, pH 8.0, vapor diffusion, hanging drop, temperature 298K