4L9G
Structure of PpsR N-Q-PAS1 from Rb. sphaeroides
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-06-17 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9785 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 50.400, 107.910, 92.060 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.549 - 2.200 |
| R-factor | 0.1795 |
| Rwork | 0.177 |
| R-free | 0.23320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PpsR Q-PAS1 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.209 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.5.0) |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.300 |
| High resolution limit [Å] | 2.200 | 10.000 | 2.200 |
| Rmerge | 0.029 | 0.631 | |
| Number of reflections | 26156 | 330 | 3183 |
| <I/σ(I)> | 10.37 | 31.87 | 2.38 |
| Completeness [%] | 99.8 | 98.5 | 99.7 |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 1.6 M ammonium sulfate, 0.1 M 2-(N-morpholino)ethanesulfonic acid, 0.01 M L-cysteine, 4% (v/v) 1,4-dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
