4L9E
Structure of PpsR Q-PAS1 from Rb. sphaeroides
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-05-04 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9765 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 50.380, 50.380, 163.310 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.630 - 1.650 |
| R-factor | 0.198 |
| Rwork | 0.197 |
| R-free | 0.21900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4l9f |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.966 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.700 |
| High resolution limit [Å] | 1.650 | 10.000 | 1.650 |
| Rmerge | 0.028 | 0.558 | |
| Number of reflections | 15633 | 101 | 1299 |
| <I/σ(I)> | 21.06 | 52.89 | 4.42 |
| Completeness [%] | 99.9 | 100 | 100 |
| Redundancy | 12.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 1.9% (w/v) PEG 3000, 0.2 M magnesium chloride, 0.1 M sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
