4L9D
Crystal structure of the PKD1 domain from Vibrio cholerae metalloprotease PrtV
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0398 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.800, 50.833, 67.297 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 12.000 - 1.100 |
| R-factor | 0.11099 |
| Rwork | 0.109 |
| R-free | 0.14040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.192 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | Auto-Rickshaw |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 12.000 | 1.150 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rmerge | 0.060 | |
| Number of reflections | 58243 | |
| <I/σ(I)> | 16.7 | |
| Completeness [%] | 99.0 | 96.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 1.2 M sodium citrate and 20% (w/v) PEG 8K, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
