4L9C

Crystal structure of the FP domain of human F-box protein Fbxo7 (native)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97872
Spacegroup name	P 21 21 21
Unit cell lengths	44.220, 64.410, 89.310
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.628 - 2.100
R-factor	0.1965
Rwork	0.191
R-free	0.24660
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.857
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall
High resolution limit [Å]	2.100
Number of reflections	15476

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	PEG 3350, MES 7.2, KBr , pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K