4L9B
Structure of native RBP from lactococcal phage 1358 (CsI derivative)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-12-12 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.55 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 107.610, 107.610, 107.610 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.360 - 1.750 |
| R-factor | 0.1708 |
| Rwork | 0.169 |
| R-free | 0.20130 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.100 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXS |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.046 | 0.830 |
| Number of reflections | 41979 | |
| <I/σ(I)> | 24.3 | 2 |
| Completeness [%] | 99.7 | 98.2 |
| Redundancy | 9 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 293 | 20% w/v PEG1000, 100 mM sodium/potassium phosphate, pH 6.2, 200 mM sodium chloride, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
