Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-11-07 |
Detector | ADSC QUANTUM 4r |
Wavelength(s) | 0.97898, 0.97926, 0.96403 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 43.504, 135.958, 51.568 |
Unit cell angles | 90.00, 92.51, 90.00 |
Refinement procedure
Resolution | 41.399 - 2.000 |
R-factor | 0.1666 |
Rwork | 0.165 |
R-free | 0.20530 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 0.996 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.000 |
Number of reflections | 79331 |
Completeness [%] | 99.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.2M Ammonium sulfate, 0.1M BIS-TRIS pH6.5, 25% w/v PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 293K |