4L97
Structure of the RBP of lactococcal phage 1358 in complex with glucose-1-phosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-03-08 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.921 |
Spacegroup name | P 21 3 |
Unit cell lengths | 165.550, 165.550, 165.550 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.790 - 2.610 |
R-factor | 0.1805 |
Rwork | 0.179 |
R-free | 0.21130 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4l9b |
RMSD bond length | 0.010 |
RMSD bond angle | 1.170 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | BUSTER (2.11.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.680 |
High resolution limit [Å] | 2.610 | 2.610 |
Rmerge | 0.106 | 0.700 |
Number of reflections | 45762 | |
<I/σ(I)> | 10.6 | 2.4 |
Completeness [%] | 99.4 | 98.6 |
Redundancy | 5.4 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 10 mM zinc sulfate heptahydrate, 100 mM MES, pH 6.5, 25% v/v PEG550 MME, VAPOR DIFFUSION, SITTING DROP, temperature 293K |