4L92

Structure of the RBP from lactococcal phage 1358 in complex with 2 GlcNAc molecules

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-02-07
Detector	PSI PILATUS 6M
Wavelength(s)	0.921
Spacegroup name	P 21 3
Unit cell lengths	165.640, 165.640, 165.640
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.750 - 2.100
R-factor	0.1772
Rwork	0.176
R-free	0.19340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4l9b
RMSD bond length	0.010
RMSD bond angle	1.120
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	BUSTER (2.11.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.230
High resolution limit [Å]	2.100	2.100
Rmerge	0.099	0.700
Number of reflections	88185
<I/σ(I)>	14.8	2.3
Completeness [%]	99.9	99.7
Redundancy	10	10

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.2	293	10 mM zinc sulfate heptahydrate, 100 mM MES, pH 6.5, 25% v/v PEG550 MME, VAPOR DIFFUSION, SITTING DROP, temperature 293K