4L8L
Crystal Structure of the Type II Dehydroquinase from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 170 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-01-03 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | F 2 3 |
| Unit cell lengths | 125.390, 125.390, 125.390 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.350 - 1.740 |
| R-factor | 0.16268 |
| Rwork | 0.161 |
| R-free | 0.19185 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.923 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.350 | 1.800 |
| High resolution limit [Å] | 1.736 | 1.740 |
| Rmerge | 0.100 | 0.215 |
| Number of reflections | 16975 | |
| <I/σ(I)> | 31.2 | 3.5 |
| Completeness [%] | 99.0 | 100 |
| Redundancy | 41.8 | 6.96 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.8M potassium sodium tartarate and 0.1M Sodium HEPES pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
