4L8J

Crystal structure of a Putative efflux transporter (BACEGG_01895) from Bacteroides eggerthii DSM 20697 at 2.06 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-05-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97871, 0.91837, 0.97817
Spacegroup name	P 41 21 2
Unit cell lengths	69.536, 69.536, 187.676
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.486 - 2.060
R-factor	0.2093
Rwork	0.208
R-free	0.23440
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.040
Data reduction software	XDS
Data scaling software	XSCALE (July 4, 2012)
Phasing software	SHELX
Refinement software	BUSTER-TNT (2.10.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.486		2.130
High resolution limit [Å]	2.060	4.420	2.060
Rmerge	0.076	0.037	0.884
Number of reflections	29410	3239	2727
<I/σ(I)>	14.77	37.9	2.2
Completeness [%]	99.8	99.2	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9.83	277	12.0% polyethylene glycol 8000, 0.1M sodium chloride, 0.1M CAPS pH 9.83, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K