4L85

Crystal structure of receiver domain of KdpE D52A mutant from E. coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2012-03-18
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.54984
Spacegroup name	P 31 2 1
Unit cell lengths	108.918, 108.918, 74.408
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.210 - 2.202
R-factor	0.176
Rwork	0.174
R-free	0.21910
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.102
Data reduction software	HKL-2000
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: dev_1352))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.210	2.280
High resolution limit [Å]	2.200	2.200
Number of reflections	26052
<I/σ(I)>		6.08
Completeness [%]	99.9	100
Redundancy	21.8	21.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	15% PEG 8K, 0.1M NaCl, 0.1M TRIS pH 7.2-8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K