4L85
Crystal structure of receiver domain of KdpE D52A mutant from E. coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-03-18 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.54984 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 108.918, 108.918, 74.408 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.210 - 2.202 |
R-factor | 0.176 |
Rwork | 0.174 |
R-free | 0.21910 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.102 |
Data reduction software | HKL-2000 |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: dev_1352)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.210 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 26052 | |
<I/σ(I)> | 6.08 | |
Completeness [%] | 99.9 | 100 |
Redundancy | 21.8 | 21.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 15% PEG 8K, 0.1M NaCl, 0.1M TRIS pH 7.2-8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |