4L83

Structure of a putative Ubiquitin-conjugating enzyme E2 from Brugia malayi

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2013-04-03
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97857
Spacegroup name	P 21 2 21
Unit cell lengths	34.850, 52.030, 108.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.970 - 1.700
R-factor	0.172
Rwork	0.170
R-free	0.20700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2grn
RMSD bond length	0.009
RMSD bond angle	1.338
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.5.2)
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000		1.740
High resolution limit [Å]	1.700	7.600	1.700
Rmerge	0.056	0.040	0.424
Number of reflections	22536	308	1665
<I/σ(I)>	19.48	35.67	3.92
Completeness [%]	99.9	97.5	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	28mg/ml BrmaA.00250.a, 200mM sodium malonate dibasic, 20% PEG3350, 20% ethylene glycol for cryoprotection. , pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 298K