4L7X
Crystal structure of the DIDO PHD finger in complex with H3K4me3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-02-26 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.000 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 40.820, 40.820, 80.620 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.682 - 1.350 |
R-factor | 0.1329 |
Rwork | 0.132 |
R-free | 0.14220 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.169 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.700 | 1.400 |
High resolution limit [Å] | 1.350 | 1.350 |
Number of reflections | 28593 | |
Completeness [%] | 100.0 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 0.1M HEPES pH8, 30% PEG6000, VAPOR DIFFUSION, HANGING DROP, temperature 291K |