4L7W
Crystal structure mutant H77A of human HD domain-containing protein 2, Genomics Consortium (NESG) Target HR6723
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-11-06 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.979 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 71.380, 71.380, 199.308 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.690 - 2.305 |
R-factor | 0.1763 |
Rwork | 0.174 |
R-free | 0.22810 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4dmb |
RMSD bond length | 0.008 |
RMSD bond angle | 1.101 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | BALBES |
Refinement software | PHENIX (dev_1269) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.300 |
Rmerge | 0.096 |
Number of reflections | 11866 |
<I/σ(I)> | 40.9 |
Completeness [%] | 100.0 |
Redundancy | 8.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | microbatch under oil | 8 | 277 | Protein solution: 100mM NaCl, 5mM DTT, 0.02% NaN3, 10mM Tris-HCl (pH 7.5) . Reservoir solution: 0.1M Mg(NO3)2, 0.1M Tris, 12% PEG 20000, microbatch under oil, temperature 277K |