4L6V
Crystal structure of a virus like photosystem I from the cyanobacterium Synechocystis PCC 6803
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-11-03 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 214.619, 133.680, 219.846 |
| Unit cell angles | 90.00, 111.14, 90.00 |
Refinement procedure
| Resolution | 29.996 - 3.800 |
| R-factor | 0.255 |
| Rwork | 0.253 |
| R-free | 0.29720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4kt0 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.771 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2-1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 4.000 |
| High resolution limit [Å] | 3.800 | 3.800 |
| Rmerge | 0.116 | 1.238 |
| Number of reflections | 113221 | |
| <I/σ(I)> | 1.2 | |
| Completeness [%] | 98.9 | 99.5 |
| Redundancy | 3.8 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 30mM Tricine-NaOH, 90mM NaCl, 90mM MgCl2, 100mM Glycine, 0.005% nonyl beta maltoside., pH 8, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
