4L6U
Crystal structure of AF1868: Cmr1 subunit of the Cmr RNA silencing complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-09-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.2000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.695, 64.162, 79.278 |
| Unit cell angles | 90.00, 93.79, 90.00 |
Refinement procedure
| Resolution | 79.100 - 2.500 |
| R-factor | 0.2313 |
| Rwork | 0.230 |
| R-free | 0.25330 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.702 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.100 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.058 | 0.673 |
| Number of reflections | 24479 | |
| <I/σ(I)> | 1.4 | |
| Completeness [%] | 99.1 | 98.8 |
| Redundancy | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.5 | 295 | 20%(w/v) polyethyleneglycol 6000, 0.1M NaCl, 0.1M Bis-Tris, 60mM beta-mercaptoethanol, pH 5.5, VAPOR DIFFUSION, temperature 295K |
