4L6P

Structure of C22Y Mutant PCNA protein defective in DNA mismatch repair

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	85.941, 90.619, 140.580
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.914 - 2.680
R-factor	0.2134
Rwork	0.210
R-free	0.27960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1plq
RMSD bond length	0.010
RMSD bond angle	1.330
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.914	2.780
High resolution limit [Å]	2.680	2.680
Number of reflections	31494
Completeness [%]	100.0	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	2M AmSO4, 0.2M Lithium Sulfate Monohydrate, 0.1M Sodium Cacodylate Trihydrate, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 291K