4L69
Rv2372c of Mycobacterium tuberculosis is RsmE like methyltransferse
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-05-18 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9762 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 81.940, 81.940, 115.070 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 51.750 - 2.900 |
R-factor | 0.2009 |
Rwork | 0.199 |
R-free | 0.23570 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1vhy |
RMSD bond length | 0.008 |
RMSD bond angle | 1.090 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | BALBES |
Refinement software | BUSTER (2.10.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 57.940 | |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.067 | |
Number of reflections | 8836 | |
Completeness [%] | 99.8 | 99.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 297 | pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 297K |