4L68
Structure of the psedudokinase domain of BIR2, an immune regulator of the RLK/Pelle family
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-04-30 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.07 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 108.141, 108.141, 392.655 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.122 - 2.000 |
| R-factor | 0.177 |
| Rwork | 0.175 |
| R-free | 0.21330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.982 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CCP4 |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.122 | 2.190 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 60269 | |
| Completeness [%] | 100.0 | 23.52 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.03 M n-ethylene glycols, 0.1 M bicine/Trizma base, 10% PEG20000, 20% PEG550 MME, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
