4L68
Structure of the psedudokinase domain of BIR2, an immune regulator of the RLK/Pelle family
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-04-30 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.07 |
Spacegroup name | H 3 2 |
Unit cell lengths | 108.141, 108.141, 392.655 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.122 - 2.000 |
R-factor | 0.177 |
Rwork | 0.175 |
R-free | 0.21330 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 0.982 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | CCP4 |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.122 | 2.190 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 60269 | |
Completeness [%] | 100.0 | 23.52 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.03 M n-ethylene glycols, 0.1 M bicine/Trizma base, 10% PEG20000, 20% PEG550 MME, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |