4L63
Apo form of AB5 holotoxin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.953699 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 120.530, 120.530, 273.510 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.211 - 1.800 |
| R-factor | 0.1781 |
| Rwork | 0.177 |
| R-free | 0.19730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wv6 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.093 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 39.500 |
| High resolution limit [Å] | 1.800 |
| Rmerge | 0.072 |
| Number of reflections | 109241 |
| <I/σ(I)> | 8.2 |
| Completeness [%] | 99.9 |
| Redundancy | 11.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 295 | 0.1M HEPES and 0.6M Na/K/PO4, VAPOR DIFFUSION, HANGING DROP, temperature 295K, pH 4.5 |
