4L61

Crystal structure of the Candida albicans Methionine Synthase in complex with Methionine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	CCD
Collection date	2012-05-13
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97648
Spacegroup name	P 21 21 21
Unit cell lengths	77.490, 97.216, 97.862
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.980 - 2.130
R-factor	0.17867
Rwork	0.176
R-free	0.23119
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.713
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.000	2.170
High resolution limit [Å]	2.100	2.130
Rmerge	0.097	0.570
Number of reflections	42001
<I/σ(I)>	8.7	3.1
Completeness [%]	100.0	100
Redundancy	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	293	50 mM NaI, 27% (w/v) PEG 3350, 0.25 mM DTT, 0.15 mM ZnSO4, 10 mM Methionine, 20 mM Tris-Cl pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K